MicroRNA-2400 promotes bovine preadipocyte proliferation

Biochem Biophys Res Commun. 2016 Sep 23;478(3):1054-9. doi: 10.1016/j.bbrc.2016.08.038. Epub 2016 Aug 8.

Abstract

MicroRNAs (miRNAs) play critical roles in the proliferation of bovine preadipocytes. miR-2400 is a novel and unique miRNA from bovines. In the present study, we separated and identified preadipocytes from bovine samples. miR-2400 overexpression increased the rate of preadipocyte proliferation, which was analyzed with a combination of EdU and flow cytometry. Simultaneously, functional genes related to proliferation (PCNA, CCND2, CCNB1) were also increased, which was detected by real-time PCR. Furthermore, luciferase reporter assays showed that miR-2400 bound directly to the 3'untranslated regions (3'UTRs) of PRDM11 mRNA. These data suggested that miR-2400 could promote preadipocyte proliferation by targeting PRDM11.

Keywords: Bovine; PRDM11; Preadipocytes; Proliferation; miR-2400.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Adipocytes / cytology*
  • Adipocytes / metabolism*
  • Animals
  • Animals, Newborn
  • Base Sequence
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Cattle
  • Cell Differentiation / genetics
  • Cell Proliferation
  • Cell Separation
  • Down-Regulation / genetics
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • 3' Untranslated Regions
  • Carrier Proteins
  • MicroRNAs
  • RNA, Messenger