The Chromosome Microdissection and Microcloning Technique

Ying-Xin Zhang; Chuan-Liang Deng; Zan-Min Hu

doi:10.1007/978-1-4939-3622-9_12

The Chromosome Microdissection and Microcloning Technique

Methods Mol Biol. 2016:1429:151-60. doi: 10.1007/978-1-4939-3622-9_12.

Authors

Ying-Xin Zhang^{1

2}, Chuan-Liang Deng³, Zan-Min Hu⁴

Affiliations

¹ Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, 100101, China.
² Graduate University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
³ Henan Normal University, Xinxiang, 453007, China.
⁴ Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, 100101, China. zmhu@genetics.ac.cn.

PMID: 27511173
DOI: 10.1007/978-1-4939-3622-9_12

Abstract

Chromosome microdissection followed by microcloning is an efficient tool combining cytogenetics and molecular genetics that can be used for the construction of the high density molecular marker linkage map and fine physical map, the generation of probes for chromosome painting, and the localization and cloning of important genes. Here, we describe a modified technique to microdissect a single chromosome, paint individual chromosomes, and construct single-chromosome DNA libraries.

Keywords: Chromosome microdissection and microcloning; Degenerated oligonucleotide-primed-PCR; Fluorescence in situ hybridization; Single-chromosome library.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Painting / methods
Chromosomes, Plant*
Cloning, Molecular / methods*
Gene Library*
In Situ Hybridization, Fluorescence / methods
Microdissection / methods*
Polymerase Chain Reaction / methods
Secale / genetics*