Dissecting Plant Chromosomes by the Use of Ionizing Radiation

Penny M A Kianian; Katie L Liberatore; Marisa E Miller; Justin B Hegstad; Shahryar F Kianian

doi:10.1007/978-1-4939-3622-9_8

Dissecting Plant Chromosomes by the Use of Ionizing Radiation

Methods Mol Biol. 2016:1429:91-101. doi: 10.1007/978-1-4939-3622-9_8.

Authors

Penny M A Kianian¹, Katie L Liberatore², Marisa E Miller³, Justin B Hegstad⁴, Shahryar F Kianian⁵

Affiliations

¹ Department of Horticultural Science, University of Minnesota, 1970 Folwell Ave., St. Paul, MN, 55108, USA. kiani002@umn.edu.
² USDA-ARS, Cereal Disease Laboratory, Department of Plant Pathology, University of Minnesota, 1551 Lindig Ave., St. Paul, MN, 55108, USA.
³ Department of Horticultural Science, University of Minnesota, 1970 Folwell Ave., St. Paul, MN, 55108, USA.
⁴ Department of Plant Sciences, North Dakota State University, Fargo, ND, USA.
⁵ USDA-ARS, Cereal Disease Laboratory, Department of Plant Pathology, University of Minnesota, 1551 Lindig Ave., St. Paul, MN, 55108, USA. shahryar.kianian@ars.usda.gov.

PMID: 27511169
DOI: 10.1007/978-1-4939-3622-9_8

Abstract

Radiation treatment of genomes is used to generate chromosome breaks for numerous applications. This protocol describes the preparation of seeds and the determination of the optimal level of irradiation dosage for the creation of a radiation hybrid (RH) population. These RH lines can be used to generate high-resolution physical maps for the assembly of sequenced genomes as well as the fine mapping of genes. This procedure can also be used for mutation breeding and forward/reverse genetics.

Keywords: Gamma rays; Gene cloning; Mapping; Mutation; Radiation hybrid; Tempering seed.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Chromosomes, Plant / radiation effects*
Genome, Plant*
Radiation Hybrid Mapping / methods*
Radiation, Ionizing*
Triticum / genetics*
Triticum / radiation effects