Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on the Genotoxicity and Heat Shock Protein (Hsp) Expression of Cells Derived from Human Eye

Shin Koyama; Eijiro Narita; Yoko Shimizu; Yukihisa Suzuki; Takeo Shiina; Masao Taki; Naoki Shinohara; Junji Miyakoshi

doi:10.3390/ijerph13080802

Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on the Genotoxicity and Heat Shock Protein (Hsp) Expression of Cells Derived from Human Eye

Int J Environ Res Public Health. 2016 Aug 8;13(8):802. doi: 10.3390/ijerph13080802.

Authors

Shin Koyama¹, Eijiro Narita², Yoko Shimizu³, Yukihisa Suzuki⁴, Takeo Shiina⁵, Masao Taki⁶, Naoki Shinohara⁷, Junji Miyakoshi⁸

Affiliations

¹ Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. shin_koyama@rish.kyoto-u.ac.jp.
² Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. narita.eijirou.4e@kyoto-u.ac.jp.
³ Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. yoko_shimizu@rish.kyoto-u.ac.jp.
⁴ Department of Electrical & Electronic Engineering, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1, Hachioji, Tokyo 192-0397, Japan. y_suzuki@tmu.ac.jp.
⁵ Department of Electrical & Electronic Engineering, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1, Hachioji, Tokyo 192-0397, Japan. shiina-takeo@ed.tmu.ac.jp.
⁶ Department of Electrical & Electronic Engineering, Graduate Schools of Science and Engineering, Tokyo Metropolitan University, 1-1, Hachioji, Tokyo 192-0397, Japan. masao@tmu.ac.jp.
⁷ Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. shino@rish.kyoto-u.ac.jp.
⁸ Laboratory of Applied Radio Engineering for Humanosphere, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto 611-0011, Japan. miyakoshi@rish.kyoto-u.ac.jp.

Abstract

Human corneal epithelial (HCE-T) and human lens epithelial (SRA01/04) cells derived from the human eye were exposed to 60 gigahertz (GHz) millimeter-wavelength radiation for 24 h. There was no statistically significant increase in the micronucleus (MN) frequency in cells exposed to 60 GHz millimeter-wavelength radiation at 1 mW/cm² compared with sham-exposed controls and incubator controls. The MN frequency of cells treated with bleomycin for 1 h provided positive controls. The comet assay, used to detect DNA strand breaks, and heat shock protein (Hsp) expression also showed no statistically significant effects of exposure. These results indicate that exposure to millimeter-wavelength radiation has no effect on genotoxicity in human eye cells.

Keywords: cellular genotoxicity; comet assay; heat shock protein; human eye cells; long-term exposure; micronucleus formation; millimeter-waves.

MeSH terms

Bleomycin
Cell Line / radiation effects*
Comet Assay
DNA Damage / radiation effects*
Eye*
Heat-Shock Proteins / metabolism*
Humans
Lens, Crystalline
Microwaves
Radiation Exposure / adverse effects*
Radio Waves / adverse effects*

Substances

Heat-Shock Proteins
Bleomycin