Seeing is Believing: Tracking Translation Dynamics In Vivo

Chunhua Li; Yan Tian; Xianghui Fu

doi:10.1016/j.tibs.2016.07.012

Seeing is Believing: Tracking Translation Dynamics In Vivo

Trends Biochem Sci. 2016 Oct;41(10):818-821. doi: 10.1016/j.tibs.2016.07.012. Epub 2016 Aug 5.

Authors

Chunhua Li¹, Yan Tian¹, Xianghui Fu²

Affiliations

¹ Division of Endocrinology and Metabolism, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, Sichuan, China.
² Division of Endocrinology and Metabolism, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, Sichuan, China. Electronic address: xfu@scu.edu.cn.

PMID: 27503842
DOI: 10.1016/j.tibs.2016.07.012

Abstract

Translation is a crucial step in transmitting genetic information from mRNA to functional protein. Four recent papers provide novel imaging approaches to directly observe translation of single mRNAs in real time in live cells, and represent a powerful toolkit to dissect the molecular mechanisms of translation regulation.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Fluorescent Dyes / chemistry
HEK293 Cells
Humans
Immunoglobulin Fab Fragments / chemistry
Molecular Imaging / methods*
Protein Biosynthesis*
RNA, Messenger / genetics*
RNA, Messenger / metabolism
Ribosomes / chemistry*
Staining and Labeling / methods
Time-Lapse Imaging

Substances

Fluorescent Dyes
Immunoglobulin Fab Fragments
RNA, Messenger