Simultaneous determination of 6-mercaptopruine, 6-thioguanine and dasatinib as three important anticancer drugs using nanostructure voltammetric sensor employing Pt/MWCNTs and 1-butyl-3-methylimidazolium hexafluoro phosphate

Hassan Karimi-Maleh; Abdollah Fallah Shojaei; Khalil Tabatabaeian; Fatemeh Karimi; Shahryar Shakeri; Reza Moradi

doi:10.1016/j.bios.2016.07.086

Simultaneous determination of 6-mercaptopruine, 6-thioguanine and dasatinib as three important anticancer drugs using nanostructure voltammetric sensor employing Pt/MWCNTs and 1-butyl-3-methylimidazolium hexafluoro phosphate

Biosens Bioelectron. 2016 Dec 15:86:879-884. doi: 10.1016/j.bios.2016.07.086. Epub 2016 Jul 26.

Authors

Hassan Karimi-Maleh¹, Abdollah Fallah Shojaei², Khalil Tabatabaeian³, Fatemeh Karimi⁴, Shahryar Shakeri⁵, Reza Moradi⁶

Affiliations

¹ Department of Chemistry, Graduate University of Advanced Technology, Kerman, Iran. Electronic address: h.karimi.maleh@gmail.com.
² Department of Chemistry, Faculty of Sciences, University of Guilan, P.O. Box 1914, Rasht, Iran. Electronic address: shoja47@gmail.com.
³ Department of Chemistry, Faculty of Sciences, University of Guilan, P.O. Box 1914, Rasht, Iran.
⁴ Pardis Compus, University of Guilan, P.O. Box 1841, Rasht, Iran.
⁵ Department of Biotechnology, Inst. of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
⁶ Department of Physics, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran.

PMID: 27494812
DOI: 10.1016/j.bios.2016.07.086

Abstract

6-Mercaptopurine, 6-thioguanine and dasatinib are three important anticancer drugs with high adverse effects in human body. In this study, a Pt/MWCNTs-1-butyl-3-methylimidazolium hexafluoro phosphate-modified carbon paste electrode was developed for the simultaneously determination of 6-mercaptopurine, 6-thioguanine and dasatinib for the first time. The Pt/MWCNTs synthesized by polyol method and have been characterized by transmission electron microscopy and X-ray diffraction methods. The obtained data revealed that the electro-oxidation of 6-mercaptopurine, 6-thioguanine and dasatinib is facilitated as a novel voltammetric sensor. After optimization of electrochemical parameters employing this sensor at pH 8.0, the oxidation peak currents for 6-mercaptopurine, 6-thioguanine and dasatinib were found to vary linearly with their concentrations in the range of 0.05-550μM; 0.1-500μM and 5.0-500μM with detection limits of 0.009μM, 0.05μM and 1.0μM respectively using square wave voltammetric method. The modified electrode has been applied for the selective and precise analysis of 6-mercaptopurine, 6-thioguanine and dasatinib in pharmaceutical formulations and urine samples.

Keywords: 6-Mercaptopurine; 6-Thioguanine; Dasatinib; Ionic liquid; Pt/MWCNTs; Sensor.

MeSH terms

Antineoplastic Agents / analysis
Antineoplastic Agents / chemistry
Antineoplastic Agents / urine
Complex Mixtures / analysis
Complex Mixtures / chemistry
Conductometry / instrumentation*
Dasatinib / analysis*
Dasatinib / chemistry
Dasatinib / urine
Equipment Design
Equipment Failure Analysis
Humans
Imidazoles / chemistry*
Mercaptopurine / analysis*
Mercaptopurine / chemistry
Mercaptopurine / urine
Metal Nanoparticles / chemistry
Metal Nanoparticles / ultrastructure
Nanotubes, Carbon / chemistry*
Nanotubes, Carbon / ultrastructure
Platinum / chemistry
Reproducibility of Results
Sensitivity and Specificity
Thioguanine / analysis*
Thioguanine / chemistry
Thioguanine / urine

Substances

1-butyl-3-methylimidazolium
Antineoplastic Agents
Complex Mixtures
Imidazoles
Nanotubes, Carbon
Platinum
Mercaptopurine
Thioguanine
Dasatinib