Inducing and Evaluating Skeletal Muscle Injury by Notexin and Barium Chloride

Methods Mol Biol. 2016:1460:53-60. doi: 10.1007/978-1-4939-3810-0_5.

Abstract

Models of skeletal muscle injury in animal models are invaluable tools to assess muscle stem cell (MuSC)-mediated tissue repair. The optimization and comprehensive evaluation of these approaches have greatly improved our ability to assess MuSC regenerative potential. Here we describe the procedures for skeletal muscle injury with notexin and BaCl2 and assessment of the dynamics of tissue regeneration.

Keywords: Muscle regeneration; Skeletal muscle injury.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Barium Compounds / adverse effects*
  • Chlorides / adverse effects*
  • Disease Models, Animal
  • Elapid Venoms / adverse effects*
  • Immunohistochemistry / methods
  • Mice
  • Muscle, Skeletal / drug effects*
  • Muscle, Skeletal / pathology*
  • Muscular Diseases / chemically induced*
  • Muscular Diseases / pathology*
  • Regeneration

Substances

  • Barium Compounds
  • Chlorides
  • Elapid Venoms
  • barium chloride
  • notexin