Phylogeny of tick-derived Crimean-Congo hemorrhagic fever virus strains in Iran

Peyvand Biglari; Sadegh Chinikar; Hamid Belqeiszadeh; Zakkyeh Telmadarraiy; Ehsan Mostafavi; Masoud Ghaffari; Siavash Javaherizadeh; Norbert Nowotny; Anthony R Fooks; Nariman Shahhosseini

doi:10.1016/j.ttbdis.2016.07.012

Phylogeny of tick-derived Crimean-Congo hemorrhagic fever virus strains in Iran

Ticks Tick Borne Dis. 2016 Oct;7(6):1216-1221. doi: 10.1016/j.ttbdis.2016.07.012. Epub 2016 Jul 22.

Authors

Affiliations

¹ Faculty of Modern Medical Science, Biology Biosystematic department, Islamic Azad University, Tehran Medical Science Branch, Iran. Electronic address: peyvand@pasteur.ac.ir.
² Pasteur Institute of Iran, Iran.
³ Faculty of Modern Medical Science, Biology Biosystematic department, Islamic Azad University, Tehran Medical Science Branch, Iran.
⁴ Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
⁵ Department of Epidemiology, Pasteur Institute of Iran, Iran.
⁶ Department of Veterinary, Tehran University, Iran.
⁷ Faculty of Paramedical Sciences, Clinical Laboratory Science, Islamic Azad University, Tehran Medical Branch, Iran.
⁸ Institute of Virology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria; Department of Basic Medical Sciences, College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai Healthcare City, Dubai, United Arab Emirates.
⁹ Wildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health Agency, Woodham Lane, New Haw, Surrey, KT15 3NB, UK; Department of Clinical Infection, Microbiology and Immunology, University of Liverpool, Liverpool, UK.
¹⁰ Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Bernhard Nocht Strasse 74, Hamburg, Germany. Electronic address: shahhosseini@bnitm.de.

PMID: 27491289
DOI: 10.1016/j.ttbdis.2016.07.012

Abstract

The presence of Crimean-Congo hemorrhagic fever virus (CCHFV) in Iran was assessed by collecting ticks from Golpayegan, Isfahan Province. Real time RT-PCR was used to detect the CCHFV RNA in the tick population and the origins of the viral sequences were determined. The CCHFV RNA was detected in 5.2% of 492 ticks collected from livestock in different regions of Golpayegan. The tick species that tested positive for the presence of CCHFV RNA included Hyalomma, Rhipicephalus and Haemaphysalis species. Phylogenetic analysis using the partial S-segment indicated that eight sequences clustered in clade IV (Asia-1) and three other sequences aligned within clade VI (Europe) with other CCHFV strains from Kosovo (Kosova1917) and Russia (Kashmanov).

Keywords: CCHFV; Golpayegan; Haemaphysalis; Hyalomma; Iran; Rhipicephalus; Tick; Virus.

MeSH terms

Animals
Genetic Variation
Hemorrhagic Fever Virus, Crimean-Congo / genetics*
Iran
Ixodidae / virology*
Phylogeny*
Seasons