Zinc supplementation during in vitro maturation increases the production efficiency of cloned pigs

J Reprod Dev. 2016 Dec 20;62(6):635-638. doi: 10.1262/jrd.2016-072. Epub 2016 Aug 2.

Abstract

Zinc supplementation (0.8 µg/ml) in in vitro maturation (IVM) medium significantly enhances oocyte quality. In this study, we compared the development of somatic cell nuclear transfer (SCNT) embryos produced from conventional IVM (control) and zinc-supplemented IVM oocytes. A total of 1206 and 890 SCNT embryos were produced using control and zinc-supplemented oocytes, respectively, and then were transferred to 11 and 8 recipients, respectively. Five control recipients and three zinc-supplemented recipients became pregnant. Two live piglets and eight mummies were born from two control recipients, and ten live piglets and six stillborn piglets were born from three zinc-supplemented recipients. The production efficiency significantly increased in the zinc-supplemented group (0.33% vs. 3.02%). This report suggests that zinc supplementation in IVM medium improved the production efficiency of cloned pigs.

MeSH terms

  • Animals
  • Cloning, Organism / methods
  • Cloning, Organism / veterinary*
  • Embryonic Development / drug effects*
  • Female
  • In Vitro Oocyte Maturation Techniques / methods
  • In Vitro Oocyte Maturation Techniques / veterinary*
  • Nuclear Transfer Techniques
  • Pregnancy
  • Pregnancy Outcome
  • Swine
  • Zinc / administration & dosage*

Substances

  • Zinc