Effect of ultrasonic treatment on the physicochemical properties and antioxidant activities of polysaccharide from Cyclocarya paliurus

Abstract

The effects of ultrasonic treatment on the physicochemical properties and antioxidant activities of the polysaccharide from Cyclocarya paliurus leaves were investigated. The physicochemical properties were detected by high-performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) and fourier transform infrared spectrometer (FT-IR) as well. The antioxidant activities were evaluated by the assay of DPPH, hydroxyl free radical, β-carotene-linoleic acid, respectfully. Results showed that no considerable change was observed on the structure between the polysaccharide of Cyclocarya paliurus (CP) and the ultrasonic polysaccharide of Cyclocarya paliurus (UCP) by FT-IR, and no apparent differences were found on the content of sugar, uronic acid and protein after the ultrasonic treatment, but difference appeared in the ratio of monosaccharide composition of CP (1.0:4.6:3.5:4.5) and UCP (1.0:5.9:3.9:4.4), and the antioxidant activities of CP were promoted after ultrasonic treatment.

Keywords: 1,1-Diphenyl-2-picrylhydrazyl (PubChem CID: 74358); 1,10-Phenanthroline (PubChem CID: 72792); Antioxidant activity; Arabinose (PubChem CID: 66308); Ascorbic acid (PubChem CID: 54676536); Butylated hydroxytoluene (PubChem CID: 31404); Carbazole (PubChem CID: 6854); Coomassie brilliant blue G250 (PubChem CID: 6333920); Cyclocarya paliurus polysaccharide; Fucose (PubChem CID: 17106); Galactose (PubChem CID: 6036); Galacturonic acid (PubChem CID: 439215); Glucose (PubChem CID: 5793); Glucuronic acid (PubChem CID: 94715); Hydrogen peroxide (PubChem CID: 784); Mannose (PubChem CID: 18950); Phenol (PubChem CID: 996); Physico-chemical properties; Rhamnose (PubChem CID: 25310); Sulfuric acid (PubChem CID: 1118); Ultrasonic treatment; Xylose (PubChem CID: 135191); β-Carotene (PubChem CID: 5280489).