Bright and photostable fluorescent probe with aggregation-induced emission characteristics for specific lysosome imaging and tracking

Jiang Ouyang; Qiguang Zang; Wansong Chen; Liqiang Wang; Shuo Li; Ren-Yu Liu; Yuanyuan Deng; Zhao-Qian Liu; Juan Li; Liu Deng; You-Nian Liu

doi:10.1016/j.talanta.2016.06.031

Bright and photostable fluorescent probe with aggregation-induced emission characteristics for specific lysosome imaging and tracking

Talanta. 2016 Oct 1:159:255-261. doi: 10.1016/j.talanta.2016.06.031. Epub 2016 Jun 16.

Authors

Jiang Ouyang¹, Qiguang Zang², Wansong Chen¹, Liqiang Wang¹, Shuo Li¹, Ren-Yu Liu³, Yuanyuan Deng³, Zhao-Qian Liu⁴, Juan Li¹, Liu Deng⁵, You-Nian Liu⁶

Affiliations

¹ College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China.
² College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China; Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, Guangdong 510640, PR China.
³ Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, PR China.
⁴ Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
⁵ College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China. Electronic address: dengliu@csu.edu.cn.
⁶ College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, PR China. Electronic address: liuyounian@csu.edu.cn.

PMID: 27474306
DOI: 10.1016/j.talanta.2016.06.031

Abstract

We develop a new lysosome-targeting AIE fluorescent probe tetraphenylethene-morpholine (TPE-MPL), by incorporating a typical lysosome-targeting moiety of morpholine into a stable tetraphenylethene skeleton. Due to both the AIE and antenna effects, TPE-MPL possesses superior photostability, appreciable tolerance to microenvironment change and high lysosome targeting ability. Our findings confirm that TPE-MPL is a well-suited imaging agent for targeting lysosome and tracking dynamic movement of lysosome. Moreover, due to its synthetic accessibility, TPE-MPL could be further modified as a dual-functional probe for lysosome, thereby gain further insight into the role of lysosome in biomedical applications.

Keywords: Aggregation-induced emission; Fluorescent imaging; Lysosome-targeting; Morpholine; Tetraphenylethene.

MeSH terms

Fluorescent Dyes / metabolism*
Lysosomes / metabolism*
Optical Imaging / methods*

Substances

Fluorescent Dyes