Headspace sorptive solid phase microextraction (HS-SPME) combined with a spectrophotometry system: A simple glass devise for extraction and simultaneous determination of cyanide and thiocyanate in environmental and biological samples

H M Al-Saidi; Sami A Al-Harbi; E H Aljuhani; M S El-Shahawi

doi:10.1016/j.talanta.2016.06.019

Headspace sorptive solid phase microextraction (HS-SPME) combined with a spectrophotometry system: A simple glass devise for extraction and simultaneous determination of cyanide and thiocyanate in environmental and biological samples

Talanta. 2016 Oct 1:159:137-142. doi: 10.1016/j.talanta.2016.06.019. Epub 2016 Jun 7.

Authors

H M Al-Saidi¹, Sami A Al-Harbi¹, E H Aljuhani², M S El-Shahawi³

Affiliations

¹ Department of Chemistry, University College in Al - Jamoum, Umm Al-Qura University, 21955 Makkah, Saudi Arabia.
² Department of Chemistry, College of Applied Science, Umm Al-Qura University, P.O. Box 9168, Makkah, Saudi Arabia.
³ Department of Chemistry, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia. Electronic address: malsaeed@kau.edu.sa.

PMID: 27474290
DOI: 10.1016/j.talanta.2016.06.019

Abstract

A simple, low cost and efficient headspace sorptive solid phase microextraction (HS-SPME) method for determination of cyanide has been developed. The system comprises of a glass tube with two valves and a moveable glass slide fixed at its centre. It includes an acceptor phase polyurethane foam treated mercury (II) dithizonate [Hg(HDz)2-PUF] complex fixed inside by a septum cap in a cylindrical configuration (5.0cm length and 1.0cm diameter). The extraction is based upon the contact of the acceptor phase to the headspace and subsequently measuring the absorbance of the recovered mercury (II) dithizonate from PUFs sorbent. Unlike other HSSE, extraction and back - extractions was carried out in a closed system, thereby improving the analytical performance by preventing the analyte loss. Under the optimized conditions, a linear calibration plot in the range of 1.0-50.0µmolL(-1) was achieved with limits of detection (LOD) and quantification (LOQ) of 0.34, 1.2µmolL(-1) CN(-), respectively. Simultaneous analysis of cyanide and thiocyanate in saliva was also performed with satisfactory recoveries.

Keywords: Headspace sportive extraction; Saliva; Simultaneous determination of cyanide and thiocyanate; Wastewater.

MeSH terms

Chemistry Techniques, Analytical / instrumentation
Chemistry Techniques, Analytical / methods*
Cyanides / analysis*
Gas Chromatography-Mass Spectrometry
Glass*
Limit of Detection
Mercury / chemistry
Saliva / chemistry
Solid Phase Microextraction*
Spectrophotometry*
Thiocyanates / analysis*

Substances

Cyanides
Thiocyanates
Mercury