Positive impact of autophagy in human breast cancer cells on local immunosurveillance

Sylvain Ladoire; David Enot; Laura Senovilla; Marie Chaix; Laurence Zitvogel; Guido Kroemer

doi:10.1080/2162402X.2016.1174801

Positive impact of autophagy in human breast cancer cells on local immunosurveillance

Oncoimmunology. 2016 May 24;5(6):e1174801. doi: 10.1080/2162402X.2016.1174801. eCollection 2016 Jun.

Authors

Sylvain Ladoire¹, David Enot², Laura Senovilla³, Marie Chaix⁴, Laurence Zitvogel⁵, Guido Kroemer⁶

Affiliations

¹ Department of Medical Oncology, Georges François Leclerc Center, Dijon, France; Institut National de la Santé et de la Recherche Médicale, U1015, Equipe labellisée Ligue Nationale Contre le Cancer, Institut Gustave Roussy, Villejuif, France.
² Metabolomics and Cell Biology Platforms, Gustave Roussy Cancer Campus , Villejuif, France.
³ Equipe 11 labellisée pas la Ligue Nationale contre le Cancer, Center de Recherche des Cordeliers, Paris, France; Université Pierre et Marie Curie, Paris, France.
⁴ Department of Medical Oncology, Georges François Leclerc Center, Dijon, France; Institut National de la Santé et de la Recherche Médicale, Avenir Team, INSERM CRI-866, University of Burgundy, Dijon, France.
⁵ Institut National de la Santé et de la Recherche Médicale, U1015, Equipe labellisée Ligue Nationale Contre le Cancer, Institut Gustave Roussy, Villejuif, France; University of Paris Sud XI, Villejuif, France; Center of Clinical Investigations in Biotherapies of Cancer (CICBT), Villejuif, France.
⁶ Metabolomics and Cell Biology Platforms, Gustave Roussy Cancer Campus, Villejuif, France; Université Pierre et Marie Curie, Paris, France; Pôle de Biologie, Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, Paris, France; Department of Women's and Children's Health, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden; INSERM, U1138, Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France.

Abstract

In human breast cancer cells, the presence of cytoplasmic dots positive for microtubule-associated proteins 1A/1B light chain 3B (LC3B) indicates enhanced autophagic flux and favorable prognosis. LC3B(+) puncta within malignant cells positively correlate with the intratumoral abundance of CD8(+) cytotoxic T lymphocytes, yet negatively correlate with the frequency of local FOXP3(+) regulatory T cells and CD68(+) tumor-associated macrophages, resulting in an improvement of CD8(+)/FOXP(+) or CD8(+)/CD68(+) ratios.

Keywords: Autophagy; LC3; Treg cells.; breast cancer; lymphocytes; macrophages.