Abstract
We compared the performance of flocked and matched traditional rectal swabs collected from 236 children admitted with gastroenteritis in Botswana. All samples were tested using real time multiplex-PCR assays for nine enteric pathogens. There was a 20% higher detection of Shigella from flocked swabs, but most other pathogens had similar detection rates.
Keywords:
Flocked swabs; Gastroenteritis; McNemar exact test; Multiplex PCR; Shigella; Threshold cycle.
Copyright © 2016 Elsevier Inc. All rights reserved.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Animals
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Bacteria / classification
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Bacteria / isolation & purification
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Botswana
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Child, Preschool
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Female
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Gastroenteritis / diagnosis*
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Humans
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Infant
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Male
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Molecular Diagnostic Techniques / methods*
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Multiplex Polymerase Chain Reaction / methods*
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Parasites / classification
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Parasites / isolation & purification
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Real-Time Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Specimen Handling / methods*
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Viruses / classification
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Viruses / isolation & purification