A Novel Peptide from Soybean Protein Isolate Significantly Enhances Resistance of the Organism under Oxidative Stress

Heran Ma; Rui Liu; Ziyuan Zhao; Zhixian Zhang; Yue Cao; Yudan Ma; Yi Guo; Li Xu

doi:10.1371/journal.pone.0159938

A Novel Peptide from Soybean Protein Isolate Significantly Enhances Resistance of the Organism under Oxidative Stress

PLoS One. 2016 Jul 25;11(7):e0159938. doi: 10.1371/journal.pone.0159938. eCollection 2016.

Authors

Heran Ma¹, Rui Liu², Ziyuan Zhao¹, Zhixian Zhang¹, Yue Cao¹, Yudan Ma³, Yi Guo¹, Li Xu¹

Affiliations

¹ Key laboratory for Molecular Enzymology and Engineering, the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, Jilin, 130012, P.R. China.
² Department of endocrinology, China-Japan Union Hospital, Jilin University, Changchun, Jilin, 130033, P.R. China.
³ Sports Science Research Institute of Jilin Province, Changchun, Jilin, 130022, P. R. China.

Abstract

Recent studies have indicated that protein hydrolysates have broad biological effects. In the current study we describe a novel antioxidative peptide, FDPAL, from soybean protein isolate (SPI). The aim of this study was to purify and characterize an antioxidative peptide from SPI and determine its antioxidative mechanism. LC-MS/MS was used to isolate and identify the peptide from SPI. The sequence of the peptide was determined to be Phe-Asp-Pro-Ala-Leu (FDPAL, 561 Da). FDPAL can cause significant enhancement of resistance to oxidative stress both in cells as well as simple organisms. In Caenorhabditis elegans (C. elegans), FDPAL can up-regulate the expression of certain genes associated with resistance. The antioxidant activity of this peptide can be attributed to the presence of a specific amino acid sequence. Results from our work suggest that FDPAL can facilitate potential applications of proteins carrying this sequence in the nutraceutical, bioactive material and clinical medicine areas, as well as in cosmetics and health care products.

MeSH terms

Animals
Animals, Genetically Modified
Antioxidants / chemistry
Antioxidants / isolation & purification
Antioxidants / pharmacology*
Biological Products / chemistry
Biological Products / metabolism
Biological Products / pharmacology
Caenorhabditis elegans
Chromatography
Gene Expression Regulation
Hydrogen-Ion Concentration
Hydrolysis
Oxidation-Reduction
Oxidative Stress / drug effects*
Peptides / chemistry
Peptides / isolation & purification
Peptides / pharmacology*
Reactive Oxygen Species
Soybean Proteins / chemistry
Soybean Proteins / metabolism*
Superoxide Dismutase / genetics
Superoxide Dismutase / metabolism
Tandem Mass Spectrometry

Substances

Antioxidants
Biological Products
Peptides
Reactive Oxygen Species
Soybean Proteins
Superoxide Dismutase

Grants and funding

This study was supported by grants from the National Natural Science Foundation of China (no. 81271697, 81571791, and 31571017), the Specialized Research Fund for the Doctoral Program of Higher Education (20100061120077), and the Project of Science and Technology Department of Jilin Province, China (no. 20130206069GX). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.