Frequency domain photoacoustic and fluorescence microscopy

Biomed Opt Express. 2016 Jun 20;7(7):2692-702. doi: 10.1364/BOE.7.002692. eCollection 2016 Jul 1.

Abstract

We report on simultaneous frequency domain optical-resolution photoacoustic and fluorescence microscopy with sub-µm lateral resolution. With the help of a blood smear, we show that photoacoustic and fluorescence images provide complementary information. Furthermore, we compare theoretically predicted signal-to-noise ratios of sinusoidal modulation in frequency domain with pulsed excitation in time domain.

Keywords: (110.5125) Photoacoustics; (140.2020) Diode lasers; (170.3880) Medical and biological imaging; (170.5120) Photoacoustic imaging; (180.2520) Fluorescence microscopy.