Microbial metabolism of thiopurines: A method to measure thioguanine nucleotides

Ramya Movva; Michael Lobb; Páraic Ó Cuív; Timothy H J Florin; John A Duley; Iulia Oancea

doi:10.1016/j.mimet.2016.07.017

Microbial metabolism of thiopurines: A method to measure thioguanine nucleotides

J Microbiol Methods. 2016 Sep:128:102-107. doi: 10.1016/j.mimet.2016.07.017. Epub 2016 Jul 19.

Authors

Ramya Movva¹, Michael Lobb², Páraic Ó Cuív³, Timothy H J Florin⁴, John A Duley⁵, Iulia Oancea⁶

Affiliations

¹ Immunity Infection and Inflammation Program, Mater Research Institute-University of Queensland, Brisbane, Queensland 4101, Australia; Translational Research Institute, 37 Kent Street, Woolloongabba, Queensland 4102, Australia; School of Pharmacy, Griffith University, Queensland 4125, Australia. Electronic address: ramya.movva@mater.uq.edu.au.
² Inflammatory Diseases Biology & Therapeutics Program, Mater Research Institute-University of Queensland, Brisbane, Queensland 4101, Australia.
³ Translational Research Institute, 37 Kent Street, Woolloongabba, Queensland 4102, Australia; The University of Queensland Diamantina Institute, Brisbane, Queensland 4102, Australia.
⁴ Immunity Infection and Inflammation Program, Mater Research Institute-University of Queensland, Brisbane, Queensland 4101, Australia; Translational Research Institute, 37 Kent Street, Woolloongabba, Queensland 4102, Australia; School of Medicine-University of Queensland, St Lucia, Queensland 4072, Australia.
⁵ Inflammatory Diseases Biology & Therapeutics Program, Mater Research Institute-University of Queensland, Brisbane, Queensland 4101, Australia; School of Pharmacy, University of Queensland, Brisbane, Queensland 4072, Australia.
⁶ Immunity Infection and Inflammation Program, Mater Research Institute-University of Queensland, Brisbane, Queensland 4101, Australia; Translational Research Institute, 37 Kent Street, Woolloongabba, Queensland 4102, Australia; School of Medicine-University of Queensland, St Lucia, Queensland 4072, Australia. Electronic address: iulia.oancea@mater.uq.edu.au.

PMID: 27444548
DOI: 10.1016/j.mimet.2016.07.017

Abstract

Thiopurines are anti-inflammatory prodrugs. We hypothesised that bacteria may contribute to conversion to active drug. Escherichia coli strain DH5α was evaluated to determine whether it could metabolise the thiopurine drugs, thioguanine or mercaptopurine, to thioguanine nucleotides. A rapid and reliable high performance liquid chromatography (ultraviolet detection) method was developed to quantify indirectly thioguanine nucleotides, by measuring thioguanine nucleoside.

Keywords: Bacteria; Inflammatory bowel disease; Mercaptopurine; Thioguanine; Thioguanosine.

MeSH terms

Anti-Inflammatory Agents / metabolism*
Calibration
Chromatography, High Pressure Liquid*
Escherichia coli / metabolism
Mercaptopurine / metabolism
Nucleosides / analysis
Nucleotides / analysis
Thioguanine / metabolism*

Substances

Anti-Inflammatory Agents
Nucleosides
Nucleotides
Mercaptopurine
Thioguanine