Determination of the parental pronuclear origin in bovine zygotes: H3K9me3 versus H3K27me2-3

Sonia Heras; Lynn Vandenberghe; Ann Van Soom

doi:10.1016/j.ab.2016.07.020

Determination of the parental pronuclear origin in bovine zygotes: H3K9me3 versus H3K27me2-3

Anal Biochem. 2016 Oct 1:510:76-78. doi: 10.1016/j.ab.2016.07.020. Epub 2016 Jul 19.

Authors

Sonia Heras¹, Lynn Vandenberghe², Ann Van Soom²

Affiliations

¹ Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium. Electronic address: sonia.herasgarcia@ugent.be.
² Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium.

PMID: 27443957
DOI: 10.1016/j.ab.2016.07.020

Abstract

To study the dynamics of 5-methylcytosine and 5-hydroxymethylcytosine in zygotes, the parental origin of the pronuclei needs to be determined. To this end the use of the asymmetric distribution of histone modifications in pronuclei is becoming more popular. Here, we demonstrated that histone 3 lysine 27 di-tri-methylation shows a stable pattern being present in the maternal but not in the paternal pronucleus of bovine zygotes, even in late stages of pronuclear development. In contrast, the pattern of histone 3 lysine 9 tri-methylation is very variable, and therefore cannot be used to reliably determine the parental origin of bovine pronuclei.

Keywords: Bovine zygotes; Epigenetics; H3K27me2-3; H3K9me3; Immunofluorescence; Pronuclear sexing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Histones / analysis*
Histones / metabolism*
Methylation
Mitochondrial Replacement Therapy*
Zygote / chemistry*
Zygote / metabolism*

Substances

Histones