Mapping quantitative trait loci for heat tolerance at anthesis in rice using chromosomal segment substitution lines

Lei Zhao; Jianguo Lei; Yingjin Huang; Shan Zhu; Hongping Chen; Renliang Huang; Zhiqin Peng; Qinghua Tu; Xianhua Shen; Song Yan

doi:10.1270/jsbbs.15084

Mapping quantitative trait loci for heat tolerance at anthesis in rice using chromosomal segment substitution lines

Breed Sci. 2016 Jun;66(3):358-66. doi: 10.1270/jsbbs.15084. Epub 2016 May 20.

Authors

Lei Zhao¹, Jianguo Lei², Yingjin Huang³, Shan Zhu², Hongping Chen⁴, Renliang Huang⁴, Zhiqin Peng², Qinghua Tu⁵, Xianhua Shen⁴, Song Yan⁴

Affiliations

¹ Rice National Engineering Laboratory (Nanchang), Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China; College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China; Key Laboratory of Agriculture Responding to Climate Change, Nanchang 30045, China.
² Rice National Engineering Laboratory (Nanchang), Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China; College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China; Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Nanchang 330045, China.
³ College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China; Key Laboratory of Agriculture Responding to Climate Change, Nanchang 30045, China; Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Nanchang 330045, China.
⁴ Rice National Engineering Laboratory (Nanchang), Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences , Nanchang 330200 , China.
⁵ Rice National Engineering Laboratory (Nanchang), Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China; Jiangxi Seed Administration, Jiangxi Province 30046, China.

Abstract

To study the genetic basis of heat tolerance at anthesis, a set of chromosome segment substitution lines (CSSLs) derived from Sasanishiki (japonica ssp. heat susceptible) and Habataki (indica spp. heat tolerant) were used for analysis across three high temperature environments. Spikelet fertility (SF), daily flowering time (DFT) and pollen shedding level (PSL) under high temperature (HT) were assessed. Eleven related QTLs were detected, of which, two QTLs qSF (ht) 2 and qSF (ht) 4.2 for spikelet fertility were identified on chromosomes 2 and 4. Four QTLs qDFT3, qDFT8, qDFT10.1 and qDFT11 for daily flowering time were detected on chromosomes 3, 8, 10 and 11. The other five QTLs qPSL (ht) 1, qPSL (ht) 4.1, qPSL (ht) 5, qPSL (ht) 7 and qPSL (ht) 10.2 on chromosomes 1, 4, 5, 7 and 10, respectively, were found had effects both on spikelet fertility and pollen shedding level. Of the 11 QTLs, 8 were overlapped with QTLs reported by others, 3 QTLs qPSL (ht) 4.1, qPSL (ht) 7 and qPSL (ht) 10.2 identified in this study were novel. The stability of qPSL (ht) 4.1 was further verified at different temperatures, which could be used to improve the pollen shedding and pollen growth on stigma for rice heat-tolerance breeding.

Keywords: CSSLs; QTL; anthesis stage; heat tolerance; qPSLht4.1; rice (Oryza sativa L.).