Preparation of Targeted Anionic Lipid-Coated Polyplexes for MicroRNA Delivery

Xiaomeng Huang; Mengzi Zhang; Xinmei Wang; L James Lee; Robert J Lee

doi:10.1007/978-1-4939-3718-9_13

Preparation of Targeted Anionic Lipid-Coated Polyplexes for MicroRNA Delivery

Methods Mol Biol. 2016:1445:201-13. doi: 10.1007/978-1-4939-3718-9_13.

Authors

Xiaomeng Huang¹, Mengzi Zhang¹, Xinmei Wang¹, L James Lee¹, Robert J Lee²

Affiliations

¹ Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, 500 W. 12th Avenue, Columbus, OH, 43210, USA.
² Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, 500 W. 12th Avenue, Columbus, OH, 43210, USA. lee.1339@osu.edu.

PMID: 27436321
DOI: 10.1007/978-1-4939-3718-9_13

Abstract

As nonviral nucleic acid delivery vehicles, lipid nanoparticles (LNPs) have been widely used. Here we describe the synthesis and evaluation of LNPs based on targeted anionic lipid-coated polyplexs for therapeutic delivery of microRNA (miRNA) mimics. These LNPs are particularly suited for therapeutic delivery of oligonucleotide agents to leukemia cells.

Keywords: Anionic lipid; Lipid nanoparticles; Targeted delivery; microRNA delivery.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Anions / chemistry*
Cell Line
Gene Transfer Techniques
Humans
Lipids / chemical synthesis*
Lipids / chemistry
Liposomes
MicroRNAs / chemistry
MicroRNAs / genetics*
Nanoparticles

Substances

Anions
Lipids
Liposomes
MicroRNAs