Lipoplexes Strengthened by Anionic Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids and Anionic Polymers

Danielle Campiol Arruda; Anne Schlegel; Pascal Bigey; Virginie Escriou

doi:10.1007/978-1-4939-3718-9_8

Lipoplexes Strengthened by Anionic Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids and Anionic Polymers

Methods Mol Biol. 2016:1445:137-48. doi: 10.1007/978-1-4939-3718-9_8.

Authors

Danielle Campiol Arruda¹, Anne Schlegel², Pascal Bigey², Virginie Escriou³

Affiliations

¹ Dep Faculdade de Farmácia, Universidade Federal de Minas Gerais, avenida Presidente Antônio Carlos, 6627, 31270-901, Belo Horizonte, Brazil.
² UTCBS, CNRS UMR8258, INSERM U1022, Université Paris Descartes, Chimie ParisTech, 75006, Paris, France.
³ UTCBS, CNRS UMR8258, INSERM U1022, Université Paris Descartes, Chimie ParisTech, 75006, Paris, France. virginie.escriou@parisdescartes.fr.

PMID: 27436316
DOI: 10.1007/978-1-4939-3718-9_8

Abstract

RNA interference is an invaluable tool in biology to specifically silence a given gene. Synthetic duplexes of RNA oligonucleotides are widely used to induce mRNA degradation in cultured cells or in whole organisms. They have to be vectorized to reach their target site. Here, we describe the preparation of highly efficient siRNA vectors based on cationic liposomes and polyanionic polymers and their application in cultured cells to silence reporter and/or endogenous genes.

Keywords: Anionic polymer; Cationic lipid; Lipoplexes; RNA interference; siRNA delivery.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anions / chemistry
Cations / chemistry
Cell Line, Tumor
Gene Transfer Techniques
Lipids / chemistry*
Liposomes
Mice
Particle Size
Polymers / chemistry*
RNA, Small Interfering / chemistry
RNA, Small Interfering / genetics*

Substances

Anions
Cations
Lipids
Liposomes
Polymers
RNA, Small Interfering