Objective: To observe the bacterial demineralization of the enamel and dentin around composite restorations bonded with one-step and two-step self-etch adhesive systems using swept-source optical coherence tomography (SS-OCT).
Methods: Forty class V cavities (2.5-mm surface diameter, 2.0-mm maximum depth) were prepared on cervical areas of 20 human molars. The specimens were either treated with one-step adhesive (Clearfil Tri-S Bond ND Quick; TS) or two-step adhesive (Clearfil SE Bond; SE), restored with a flowable resin composite (Estelite Flow Quick). Specimens in the demineralized group were incubated for 2 weeks after Streptococcus mutans biofilm formation, while specimens in the control group were incubated for 2 weeks without biofilms. After SS-OCT observation, specimens were cut and examined under confocal laser scanning microscope (CLSM). The lesion depth (LD), interfacial gap depth (GD) and gap length (GL) obtained from SS-OCT and CLSM were analyzed by Pearson's correlation, LD by Independent-samples t-test, GD and GL by Welch t-test, the frequency of specimens with or without gap by Fisher's exact test at the 95% significant level.
Results: Specimens with interfacial gaps in the demineralized group showed significantly higher frequency than that in the control group (p<0.05). There was a significant correlation between SS-OCT and CLSM values of LD, GD and GL (p<0.05). SE showed significantly larger LD of dentin, but less GD and GL of enamel than TS (p<0.05).
Significance: SS-OCT nondestructively detected demineralization around composite restorations and interfacial gaps created by S. mutans biofilm in this in vitro model.
Keywords: Adhesive; Bacterial demineralization; Biofilm; Gap; Resin composite; SS-OCT; Secondary caries; Streptococcus mutans (S. mutans).
Copyright © 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.