Emergence and Plasmid Analysis of Klebsiella pneumoniae KP01 Carrying blaGES-5 from Guangzhou, China

Antimicrob Agents Chemother. 2016 Sep 23;60(10):6362-4. doi: 10.1128/AAC.00764-16. Print 2016 Oct.

Abstract

Klebsiella pneumoniae strain KP01 carrying blaGES-5 was identified from a patient in Guangzhou, China. High-throughput sequencing assigned blaGES-5 to a 28.5-kb nonconjugative plasmid, pGES-GZ. A 13-kb plasmid backbone sequence on pGES-GZ was found to share high sequence identities with plasmids from Gram-negative nonfermenters. A novel class 1 integron carrying a gene cassette array of orf28-orf28-blaGES-5 was identified on pGES-GZ, within which orf28 encoded a hypothetical protein possibly correlated to fosfomycin resistance.

MeSH terms

  • Aged
  • Bacterial Proteins / genetics*
  • China
  • Community-Acquired Infections / microbiology
  • Drug Resistance, Bacterial / genetics
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Integrons
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / drug effects*
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Male
  • Microbial Sensitivity Tests
  • Plasmids / genetics
  • Pneumonia, Bacterial / microbiology
  • beta-Lactamases / genetics*

Substances

  • Bacterial Proteins
  • beta-Lactamases
  • beta-lactamase GES-5, Klebsiella pneumoniae