Abstract
Calcium-imaging techniques were used to determine if mouse retinal astrocytes in situ respond to agonists of ionotropic (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, AMPA; N-methyl-D-aspartate, NMDA) and metabotropic (S-3,5-dihydroxyphenylglycine, DHPG; trans-1-amino-1,3-cyclopentanedicarboxylic acid, ACPD) glutamate receptors. In most cases we found no evidence that retinal astrocyte intracellular calcium ion concentration ([Ca(2+)]i) increased in response to these glutamate agonists. The one exception was AMPA that increased [Ca(2+)]i in some, but not all, mouse retinal astrocytes in situ. However, AMPA did not increase [Ca(2+)]i in mouse retinal astrocytes in vitro, suggesting that the effect of AMPA in situ may be indirect.
MeSH terms
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Animals
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Astrocytes / drug effects
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Astrocytes / metabolism*
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Calcium / metabolism*
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Cycloleucine / analogs & derivatives
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Cycloleucine / pharmacology
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Excitatory Amino Acid Agonists / pharmacology*
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Fura-2 / metabolism
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Methoxyhydroxyphenylglycol / analogs & derivatives
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Methoxyhydroxyphenylglycol / pharmacology
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Mice, Inbred C57BL
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N-Methylaspartate / pharmacology
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Receptors, Glutamate / metabolism*
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Retina / cytology*
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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / pharmacology
Substances
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Excitatory Amino Acid Agonists
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Receptors, Glutamate
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Cycloleucine
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1-amino-1,3-dicarboxycyclopentane
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Methoxyhydroxyphenylglycol
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N-Methylaspartate
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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
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Calcium
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Fura-2
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3,4-dihydroxyphenylglycol