Culture-Independent Detection of Nontuberculous Mycobacteria in Clinical Respiratory Samples

Gianny P Scoleri; Jocelyn M Choo; Lex E X Leong; Thomas R Goddard; Lisa Shephard; Lucy D Burr; Ivan Bastian; Rachel M Thomson; Geraint B Rogers

doi:10.1128/JCM.01410-16

Culture-Independent Detection of Nontuberculous Mycobacteria in Clinical Respiratory Samples

J Clin Microbiol. 2016 Sep;54(9):2395-8. doi: 10.1128/JCM.01410-16. Epub 2016 Jul 13.

Authors

Gianny P Scoleri¹, Jocelyn M Choo¹, Lex E X Leong¹, Thomas R Goddard², Lisa Shephard³, Lucy D Burr⁴, Ivan Bastian³, Rachel M Thomson⁵, Geraint B Rogers⁶

Affiliations

¹ SAHMRI Infection and Immunity Theme, School of Medicine, Flinders University, Adelaide, South Australia, Australia.
² Department of Respiratory and Medicine, WCH, and Paediatrics and Reproductive Health, School of Medicine, University of Adelaide, Australia.
³ Microbiology Infectious Diseases Directorate, SA Pathology, Adelaide, South Australia.
⁴ Immunity, Infection and Inflammation Program, Mater Research Institute, University of Queensland, Translational Research Institute, Wooloongabba, QLD, Australia Department of Respiratory Medicine, Mater Health Services, South Brisbane, QLD, Australia.
⁵ Gallipoli Medical Research Centre, Greenslopes Private Hospital and University of Queensland, Brisbane, Queensland, Australia.
⁶ SAHMRI Infection and Immunity Theme, School of Medicine, Flinders University, Adelaide, South Australia, Australia geraint.rogers@sahmri.com.

Abstract

Culture-based detection of nontuberculous Mycobacteria (NTM) in respiratory samples is time consuming and can be subject to overgrowth by nonmycobacterial bacteria. We describe a single-reaction TaqMan quantitative PCR assay for the direct detection of NTM species in clinical samples that is specific, sensitive, and robust.

Publication types

Evaluation Study

MeSH terms

Humans
Molecular Diagnostic Techniques / methods*
Mycobacterium Infections, Nontuberculous / diagnosis*
Nontuberculous Mycobacteria / isolation & purification*
Real-Time Polymerase Chain Reaction / methods*
Respiratory Tract Infections / diagnosis*
Sensitivity and Specificity

Grants and funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit-sectors.