Plasmonic nanohalo optical probes for highly sensitive imaging of survivin mRNA in living cells

Guang-Sheng Qian; Bin Kang; Zhuo-Lei Zhang; Xiang-Ling Li; Wei Zhao; Jing-Juan Xu; Hong-Yuan Chen

doi:10.1039/c6cc02831d

Plasmonic nanohalo optical probes for highly sensitive imaging of survivin mRNA in living cells

Chem Commun (Camb). 2016 Sep 25;52(74):11052-5. doi: 10.1039/c6cc02831d. Epub 2016 Jul 14.

Authors

Guang-Sheng Qian¹, Bin Kang, Zhuo-Lei Zhang, Xiang-Ling Li, Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen

Affiliation

¹ State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China. weizhao@nju.edu.cn xujj@nju.edu.cn.

PMID: 27412908
DOI: 10.1039/c6cc02831d

Abstract

A strategy is designed for sensitive detection of tumor biomarker survivin mRNA based on resonance Rayleigh scattering of a single AuNP nanohalo probe that couples large gold nanoparticles (L-AuNPs, 52 nm) with small AuNPs (S-AuNPs, 18 nm) through the affinity interaction between streptavidin and biotin. This core-satellite plasmon ruler is further applied to imaging survivin mRNA in living cells.

MeSH terms

Biotin / chemistry
Biotin / metabolism
Gold / chemistry
HeLa Cells
Humans
Inhibitor of Apoptosis Proteins / genetics*
Metal Nanoparticles / chemistry*
Particle Size
RNA, Messenger / chemistry
RNA, Messenger / metabolism*
Streptavidin / chemistry
Streptavidin / metabolism
Surface Plasmon Resonance*
Survivin

Substances

BIRC5 protein, human
Inhibitor of Apoptosis Proteins
RNA, Messenger
Survivin
Biotin
Gold
Streptavidin