Data on expression of lipoxygenases-5 and -12 in the normal and acetaminophen-damaged liver

Maria Suciu; Alexandra T Gruia; Dragos V Nica; Seyed M R Azghadi; Ani A Mic; Felix A Mic

doi:10.1016/j.dib.2016.03.079

Data on expression of lipoxygenases-5 and -12 in the normal and acetaminophen-damaged liver

Data Brief. 2016 Mar 31:7:1199-203. doi: 10.1016/j.dib.2016.03.079. eCollection 2016 Jun.

Authors

Maria Suciu¹, Alexandra T Gruia², Dragos V Nica³, Seyed M R Azghadi⁴, Ani A Mic⁴, Felix A Mic⁴

Affiliations

¹ Electron Microscopy Integrated Laboratory, National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath St., 400293 Cluj-Napoca, Romania; Molecular Biology and Biotechnology Department, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj 5-7 Clinicilor St., 400006 Napoca, Romania.
² Regional Center for Transplant Immunology, County Clinical Emergency Hospital Timisoara, 10 Iosif Bulbuca Blvd., 300736 Timisoara, Romania.
³ Regional Center for Transplant Immunology, County Clinical Emergency Hospital Timisoara, 10 Iosif Bulbuca Blvd., 300736 Timisoara, Romania; Faculty of Animal Sciences and Biotechnologies, Banat׳s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania", Timisoara, Romania.
⁴ Department of Functional Sciences, University of Medicine and Pharmacy "Victor Babes", 2 Eftimie Murgu Sq., 300041 Timisoara, Romania.

Abstract

Here we present additional data on the expression of lipoxygenases -5 and -12 in the normal and acetaminophen-damaged liver, which are associated with our manuscript recently published in Chemico-Biological Interactions on lipid metabolism and eicosanoid signaling pathways involved in acetaminophen-induced liver damage in a mouse model (http://dx.doi.org/10.1016/j.cbi.2015.10.019 [1]). It has been demonstrated that the expression of lipoxygenase-5 and leukotriene formation are increased in the livers of rats with carbon tetrachloride (CCl4)-induced cirrhosis (http://dx.doi.org/10.1053/gast.2000.17831 [2]). In addition, the lipoxygenase-12 is known to be expressed in the resident macrophage population of the liver (http://dx.doi.org/10.1016/S0014-5793(99)00396-8 [3]). Mice were injected with acetaminophen, and at 48 h their livers were processed for immunohistochemistry with anti-mouse lipoxygenase-5 and -12 antibodies. At the same time point, the RNA was also extracted from the liver to assess the expression of lipoxygenase-5 and -12 genes via qPCR analysis. Our results show that lipoxygenase-5 expression, but not that of lipoxygenase-12, changes significantly in the acetominophen-damaged liver.