Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30

Mohan C Manjegowda; Paridhi Singhal Gupta; Anil M Limaye

doi:10.1016/j.dib.2016.03.054

Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30

Data Brief. 2016 Mar 19:7:1015-20. doi: 10.1016/j.dib.2016.03.054. eCollection 2016 Jun.

Authors

Mohan C Manjegowda¹, Paridhi Singhal Gupta¹, Anil M Limaye¹

Affiliation

¹ Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.

Abstract

Rabbit antiserum was generated against the N-terminus of human GPR30 followed by peptide affinity purification. In this article, the methodology used and validation data are presented. The peptide affinity purified polyclonal antibody specifically detects human GPR30 in ELISA and on western blots of total protein prepared from human breast cancer cell lines.