Transcriptomic analyses of Hand2 transgenic embryos

Noriko Funato; Hiroki Kokubo; Yumiko Saga

doi:10.1016/j.gdata.2016.06.015

Transcriptomic analyses of Hand2 transgenic embryos

Genom Data. 2016 Jun 25:9:60-2. doi: 10.1016/j.gdata.2016.06.015. eCollection 2016 Sep.

Authors

Noriko Funato¹, Hiroki Kokubo², Yumiko Saga³

Affiliations

¹ Department of Signal Gene Regulation, Research Center for Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
² Division of Mammalian Development, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540, Japan; Department of Genetics, The Graduate University for Advanced Studies, Yata 1111, Mishima, Shizuoka 411-8540, Japan; Department of Cardiovascular Physiology and Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minamiku, Hiroshima 734-8551, Japan.
³ Division of Mammalian Development, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540, Japan; Department of Genetics, The Graduate University for Advanced Studies, Yata 1111, Mishima, Shizuoka 411-8540, Japan.

Abstract

In this article, we further provide the data generated for the previously published research article "Specification of jaw identity by the Hand2 transcription factor." To better understand the downstream genes of the basic helix-loop-helix transcription factor Hand2, we generated double-transgenic mice (Hand2 (NC) ) by intercrossing CAG-floxed CAT-Hand2 mice with Wnt1-Cre mice for conditional activation of Hand2 expression in the neural crest. Altered expression of Hand2 induces transformation of the upper jaw to the lower jaw in Hand2 (NC) mutant mice. This data article provides Tables detailing the differentially expressed genes between wild-type and Hand2 (NC) mutant embryos. The raw array data of our transcriptomes as generated using Affymetrix microarrays are available on the NCBI Gene Expression Omnibus (GEO) browser (Reference number GSE75805).

Keywords: CAG, the CMV early enhancer/chicken β actin; CAT, chloramphenicol acetyl transferase; Craniofacial development; Evolution; Jaw; Mandible; Neural crest; Vertebrates; aRNA, amplified RNA; bHLH, basic helix-loop-helix.