Abstract
Two new phthalide derivatives, angesinenolides A and B (1 and 2), were isolated from the roots of Angelica sinensis. Their structures were elucidated using HRMS, NMR, and X-ray crystallographic data. Compound 1 is the first example of a phthalide trimer presumably formed through two [2+2] cycloaddition reactions. Compound 2 is a unique dimeric phthalide with a peroxy bridge between C-3a and C-6. Both phthalides were evaluated for in vitro anticoagulation activities. Compound 1 reduced the level of fibrinogen (FIB). Compound 2 significantly extended thrombin time and activated partial thromboplastin time, as well as markedly reduced the content of FIB.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Angelica sinensis / chemistry*
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Anticoagulants / chemistry
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Anticoagulants / isolation & purification*
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Anticoagulants / pharmacology
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Benzofurans / chemistry
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Benzofurans / isolation & purification*
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Benzofurans / pharmacology*
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Crystallography, X-Ray
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / isolation & purification*
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Drugs, Chinese Herbal / pharmacology*
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Fibrinogen / analysis
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Fibrinogen / drug effects
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Molecular Structure
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Plant Roots / chemistry
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Thrombin / analysis
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Thrombin / drug effects
Substances
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Anticoagulants
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Benzofurans
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Drugs, Chinese Herbal
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angesinenolide A
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angesinenolide B
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phthalide
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Fibrinogen
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Thrombin