Different expression and subcellular localization of Phosphoinositide-specific Phospholipase C enzymes in differently polarized macrophages

Tania Di Raimo; Martina Leopizzi; Giorgio Mangino; Carlo Della Rocca; Rita Businaro; Lucia Longo; Vincenza Rita Lo Vasco

doi:10.1007/s12079-016-0335-9

Different expression and subcellular localization of Phosphoinositide-specific Phospholipase C enzymes in differently polarized macrophages

J Cell Commun Signal. 2016 Dec;10(4):283-293. doi: 10.1007/s12079-016-0335-9. Epub 2016 Jul 9.

Authors

Tania Di Raimo¹, Martina Leopizzi¹, Giorgio Mangino¹, Carlo Della Rocca¹, Rita Businaro¹, Lucia Longo², Vincenza Rita Lo Vasco³

Affiliations

¹ Department of Medico-Surgical Sciences and Biotechnology, Polo Pontino - Sapienza University, Latina, Italy.
² Department Sensory Organs, Faculty of Medicine and Dentistry, Policlinico Umberto I, Sapienza University of Rome, Viale dell'Università 33, 00100, Rome, Italy.
³ Department Sensory Organs, Faculty of Medicine and Dentistry, Policlinico Umberto I, Sapienza University of Rome, Viale dell'Università 33, 00100, Rome, Italy. ritalovasco@hotmail.it.

Abstract

Macrophages' phenotypic and functional diversity depends on differentiating programs related to local environmental factors. Recent interest was deserved to the signal transduction pathways acting in macrophage polarization, including the phosphoinositide (PI) system and related phospholipase C (PLC) family of enzymes. The expression panel of PLCs and the subcellular localization differs in quiescent cells compared to the pathological counterpart. We analyzed the expression of PLC enzymes in unpolarized (M0), as well as in M1 and M2 macrophages to list the expressed isoforms and their subcellular localization. Furthermore, we investigated whether inflammatory stimulation modified the basal panel of PLCs' expression and subcellular localization. All PLC enzymes were detected within both M1 and M2 cells, but not in M0 cells. M0, as well as M1 and M2 cells own a specific panel of expression, different for both genes' mRNA expression and intracellular localization of PLC enzymes. The panel of PLC genes' expression and PLC proteins' presence slightly changes after inflammatory stimulation. PLC enzymes might play a complex role in macrophages during inflammation and probably also during polarization.

Keywords: Gene expression; Inflammation; LPS; M0; M1; M2; Macrophage polarization; Nanotube; PLC; Podosome; Post-transcriptional regulation; Signal transduction.