Charaterization and immunomodulatory activities of polysaccharide isolated from Pleurotus eryngii

Int J Biol Macromol. 2016 Nov:92:30-36. doi: 10.1016/j.ijbiomac.2016.07.016. Epub 2016 Jul 5.

Abstract

A water-soluble polysaccharide (EPA-1) from Pleurotus eryngii was obtained using DEAE-52 and Sephadex G-50 columns. The properties, structure and immunomodulatory activity of EPA-1 were studied. The results demonstrated that EPA-1 was a homogeneous polysaccharide with the molecular weight of 9.97×104Da. EPA-1 consisted of Man, Glc and Gal in a molar ratio of 2.2:1.0:3.2. The characterized fragment structures of EPA-1 were found to be consisting of seven sugar residues and two branches by GC-MS, FTIR and NMR analyses. Among them, the (1→6)-linkedGal residue was the main linkage mode of EpA-1 and composed of its backbone. Activity tests indicated that EPA-1 significantly induced macrophage to release the immune activity factor of NO, TNF-α, IL-1 and IL-6 through activation of signal protein of p38, ERK, JNK in MAPKs and translocation of nuclear NF-κΒ, indicating EPA-1 to possess good immunoregulatory activity.

Keywords: Cytokine; Immunostimulation; MAPKs; NF-κB; Pleurotus eryngii; Polysaccharide.

MeSH terms

  • Animals
  • Carbohydrate Conformation
  • Cell Line, Tumor
  • Fungal Polysaccharides* / chemistry
  • Fungal Polysaccharides* / isolation & purification
  • Fungal Polysaccharides* / pharmacology
  • Immunologic Factors* / chemistry
  • Immunologic Factors* / isolation & purification
  • Immunologic Factors* / pharmacology
  • MAP Kinase Signaling System / drug effects*
  • MAP Kinase Signaling System / immunology
  • Macrophages / immunology
  • Macrophages / metabolism*
  • Male
  • Mice
  • Monokines / blood
  • Monokines / immunology
  • Nitric Oxide / immunology
  • Nitric Oxide / metabolism
  • Pleurotus / chemistry*

Substances

  • Fungal Polysaccharides
  • Immunologic Factors
  • Monokines
  • Nitric Oxide