Qualitative analysis of major constituents from Xue Fu Zhu Yu Decoction using ultra high performance liquid chromatography with hybrid ion trap time-of-flight mass spectrometry

Chunyan Fu; Zian Xia; Yonghui Liu; Hongmei Lu; Zhimin Zhang; Yang Wang; Xiaqiong Fan

doi:10.1002/jssc.201600083

Qualitative analysis of major constituents from Xue Fu Zhu Yu Decoction using ultra high performance liquid chromatography with hybrid ion trap time-of-flight mass spectrometry

J Sep Sci. 2016 Sep;39(17):3457-68. doi: 10.1002/jssc.201600083.

Authors

Chunyan Fu^{1

2}, Zian Xia³, Yonghui Liu², Hongmei Lu⁴, Zhimin Zhang¹, Yang Wang³, Xiaqiong Fan¹

Affiliations

¹ College of Chemistry and Chemical Engineering, Central South University, Hunan, Changsha, P. R. China.
² Department of Pharmacy, Shaoyang Medical College Level Specialty School, Hunan, Shaoyang, P. R. China.
³ Laboratory of Ethnopharmacology, Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Hunan, Changsha, P. R. China.
⁴ College of Chemistry and Chemical Engineering, Central South University, Hunan, Changsha, P. R. China. hongmeilu@csu.edu.cn.

PMID: 27384131
DOI: 10.1002/jssc.201600083

Abstract

Xue Fu Zhu Yu Decoction, a famous formula that has been used for treating many blood stasis-caused diseases for many centuries, comprises 11 kinds of traditional Chinese medicines. A convenient, efficient, and rapid analytical method was developed to simultaneously determine the major compounds in this decoction. An ultra-high performance liquid chromatography with hybrid ion trap time-of-flight mass spectrometry method was used to rapidly separate and detect the major constituents of the decoction. Using this technique, we identified or tentatively identified 34 compounds, including 21 flavonoids, 5 terpenoids, 3 organic acids, 2 lactones, 1 alkaloid, 1 amino acid, and 1 cyanogenic glycoside. The MS analysis of these constituents was described in detail. Findings may contribute to future metabolic and pharmacokinetic studies of this medicine.

Keywords: Major constituents; Qualitative analysis; Traditional Chinese medicine; Ultra-high performance liquid chromatography; Xue Fu Zhu Yu Decoction.

Publication types

Evaluation Study

MeSH terms

Chromatography, High Pressure Liquid / methods*
Drugs, Chinese Herbal / chemistry*
Mass Spectrometry / methods*
Medicine, Chinese Traditional
Plants, Medicinal / chemistry*

Substances

Drugs, Chinese Herbal
Xue-Fu-Zhu-Yu decoction