Osteoporosis is a common problem in aged people and those with related diseases, such as inflammatory bowel diseases. Deregulation of vitamin D metabolism plays a role in the pathogenesis of osteoporosis. Micro RNA (miR) can regulate cytokine expression in cells. This study test a hypothesis that inflammatory cytokine interleukin (IL)-13 increases miR-19a to compromise cyp27b1 (a vitamin D hydroxylase) in peripheral CD14+ cells. Bone mineral density of L2-L4 was measured in 20 patients with ulcerative colitis (UC) and 20 healthy subjects. Peripheral CD14+ cells were isolated from healthy people and patients with UC. Expression of cyp27b1 by CD14+ cells was analyzed in the presence or absence of IL-13 in the culture. We observed that bone mineral density (BMD) in UC patients was significantly lower than healthy subjects. The BMD is negatively correlated with miR-19a in peripheral CD14+ cells. MiR-19a in peripheral CD14+ cell was correlated with serum IL-13 in UC patients. Expression of cyp27b1 in peripheral CD14+ cells was correlated with miR-19a and serum IL-13 in UC patients. IL-13 suppressed cyp27b1 expression in CD14+ cells. IL-13 increased expression of miR-19a in CD14+ cells. IL-13 suppresses cyp27b1 expression in peripheral CD14+ cells via up regulating miR-19a expression. J. Cell. Biochem. 118: 376-381, 2017. © 2016 Wiley Periodicals, Inc.
Keywords: CD14 CELL; INTERLEUKIN 13; MICRO RNA; OSTEOPOROSIS; ULCERATIVE COLITIS.
© 2016 Wiley Periodicals, Inc.