Genomic analysis of the evolutionary lineage of Norovirus GII.4 from archival specimens during 1975-1987 in Tokyo

Kohji Mori; Pei-Yu Chu; Kazushi Motomura; Yoshiko Somura; Miyuki Nagano; Kana Kimoto; Tetsuya Akiba; Akemi Kai; Kenji Sadamasu

doi:10.1002/jmv.24624

Genomic analysis of the evolutionary lineage of Norovirus GII.4 from archival specimens during 1975-1987 in Tokyo

J Med Virol. 2017 Feb;89(2):363-367. doi: 10.1002/jmv.24624. Epub 2016 Jul 13.

Authors

Kohji Mori¹, Pei-Yu Chu², Kazushi Motomura³, Yoshiko Somura¹, Miyuki Nagano¹, Kana Kimoto¹, Tetsuya Akiba¹, Akemi Kai¹, Kenji Sadamasu¹

Affiliations

¹ Department of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
² Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan.
³ Research Institute of Microbial Diseases, Osaka University, Suita, Osaka, Japan.

PMID: 27380721
DOI: 10.1002/jmv.24624

Abstract

This study aimed to analyze NoV GII.4 sequences from archival specimens obtained during 1975-1987 by comparing them with reference sequences. The first NoV GII.P4_GII.4 sequence was identified in 1980. NoV GII.4 collected in 1970 had a GII.P1_GII.4 sequence. These results indicate that the GII.P4_GII.4 sequence may be the result of a recombination that might have occurred around 1980. Amino acid substitutions based on this replacement were mainly accumulated in the NTPase, p22, and RdRp regions. The emergence of GII.P4_GII.4 sequence is considered to have ended the major prevalence of NoV GII.4. J. Med. Virol. 89:363-367, 2017. © 2016 Wiley Periodicals, Inc.

Keywords: genetic variability; mutation; recombination.

MeSH terms

Amino Acid Substitution
Caliciviridae Infections / epidemiology
Caliciviridae Infections / virology*
Evolution, Molecular
Genotype*
Humans
Molecular Epidemiology
Norovirus / classification*
Norovirus / genetics*
Norovirus / isolation & purification
Recombination, Genetic
Sequence Analysis, DNA*
Tokyo / epidemiology