Expression of globular form acetylcholinesterase is not altered in P2Y1R knock-out mouse brain

Abstract

Adenosine 5'-triphosphate (ATP), a neurotransmitter and a neuromodulator, has been shown to be co-stored and co-released with acetylcholine (ACh) at the pre-synaptic vesicles in vertebrate neuromuscular junction (nmj). Several lines of studies demonstrated that binding of ATP to its corresponding P2Y₁ receptors (P2Y₁R) in muscle and neuron regulated the post-synaptic gene expressions. Indeed, the expression of acetylcholinesterase (AChE) in muscle was markedly decreased in P2Y1R^-/- (P2Y₁R knock-out) mice. In order to search for possible role of P2Y₁R in cholinergic function of the brain, the expression of globular form AChE was determined in the brain of P2Y1R^-/- mice. In contrast to that in muscle, the amounts of AChE activity, AChE catalytic subunit, structure subunit PRiMA and the amount of ACh, in the brain were not, significantly, altered, suggesting the role of P2Y₁R in neuron could have different function as that in muscle. However, the expressions of a series of neuronal development markers, i.e. neurofilaments, were reduced in P2Y1R^-/- mouse brain, indicating P2Y₁R may be involved in neuronal development process.

Keywords: ACh; AChE; Neuron growth; P2Y(1) receptor.