Dot-enzyme linked immunosorbent assay (Dot-ELISA) used for the serodiagnosis of both intestinal and extraintestinal amoebiasis, showed positivity in 94 per cent patients of amoebic liver abscess and 87.5 per cent of intestinal amoebiasis. Only one of the 18 normal controls was positive by Dot-ELISA test. This test showed good correlation with indirect haemagglutination test. As it is simple and easy to perform, the test appears to have great potential for the specific diagnosis of amoebiasis in peripheral hospitals and also at the field level where sophisticated immunodiagnostic facilities are not available.