Dual CARS and SHG image acquisition scheme that combines single central fiber and multimode fiber bundle to collect and differentiate backward and forward generated photons

Sheng Weng; Xu Chen; Xiaoyun Xu; Kelvin K Wong; Stephen T C Wong

doi:10.1364/BOE.7.002202

Dual CARS and SHG image acquisition scheme that combines single central fiber and multimode fiber bundle to collect and differentiate backward and forward generated photons

Biomed Opt Express. 2016 May 16;7(6):2202-18. doi: 10.1364/BOE.7.002202. eCollection 2016 Jun 1.

Authors

Sheng Weng¹, Xu Chen², Xiaoyun Xu², Kelvin K Wong², Stephen T C Wong¹

Affiliations

¹ Translational Biophotonics Lab, Department of Systems Medicine and Bioengineering, Houston Methodist Research Institute, Weill Cornell Medicine, Houston, Texas 77030, USA; Department of Electrical and Computer Engineering, Rice University, Houston, Texas 77005, USA.
² Translational Biophotonics Lab, Department of Systems Medicine and Bioengineering, Houston Methodist Research Institute, Weill Cornell Medicine, Houston, Texas 77030, USA.

Abstract

In coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) imaging, backward and forward generated photons exhibit different image patterns and thus capture salient intrinsic information of tissues from different perspectives. However, they are often mixed in collection using traditional image acquisition methods and thus are hard to interpret. We developed a multimodal scheme using a single central fiber and multimode fiber bundle to simultaneously collect and differentiate images formed by these two types of photons and evaluated the scheme in an endomicroscopy prototype. The ratio of these photons collected was calculated for the characterization of tissue regions with strong or weak epi-photon generation while different image patterns of these photons at different tissue depths were revealed. This scheme provides a new approach to extract and integrate information captured by backward and forward generated photons in dual CARS/SHG imaging synergistically for biomedical applications.

Keywords: (110.2350) Fiber optics imaging; (170.2150) Endoscopic imaging; (170.3880) Medical and biological imaging; (180.4315) Nonlinear microscopy; (190.4160) Multiharmonic generation; (300.6230) Spectroscopy, coherent anti-Stokes Raman scattering.