Monitoring and determination of sulfonamide antibiotics (sulfamethoxydiazine, sulfamethazine, sulfamethoxazole and sulfadiazine) in imported Pangasius catfish products in Thailand using liquid chromatography coupled with tandem mass spectrometry

Worawat Jansomboon; Suwanna Kitpati Boontanon; Narin Boontanon; Chongrak Polprasert; Chau Thi Da

doi:10.1016/j.foodchem.2016.06.026

Monitoring and determination of sulfonamide antibiotics (sulfamethoxydiazine, sulfamethazine, sulfamethoxazole and sulfadiazine) in imported Pangasius catfish products in Thailand using liquid chromatography coupled with tandem mass spectrometry

Food Chem. 2016 Dec 1:212:635-40. doi: 10.1016/j.foodchem.2016.06.026. Epub 2016 Jun 11.

Authors

Worawat Jansomboon¹, Suwanna Kitpati Boontanon², Narin Boontanon³, Chongrak Polprasert⁴, Chau Thi Da⁵

Affiliations

¹ Department of Civil and Environmental Engineering, Faculty of Engineering, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand.
² Department of Civil and Environmental Engineering, Faculty of Engineering, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand. Electronic address: suwanna.boo@mahidol.ac.th.
³ Faculty of Environment and Resource Studies, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand.
⁴ Department of Civil Engineering, Faculty of Engineering, Thammasat University, Khlong Luang, Pathum Thani 12120, Thailand.
⁵ Aquaculture Department, Faculty of Agriculture and Natural Resources, An Giang University, Long Xuyen, An Giang, Viet Nam.

PMID: 27374578
DOI: 10.1016/j.foodchem.2016.06.026

Abstract

This research aimed to monitor the concentrations of sulfamethoxydiazine (SMD), sulfamethazine (SMT), sulfamethoxazole (SMX) and sulfadiazine (SDZ) in imported Pangasius catfish products in Thailand. The residues of the four sulfonamides (SAs) were analyzed by extraction process and liquid chromatography coupled with tandem mass spectrometry. The highest concentrations found were 10.97ng/g for SMD, 6.23ng/g for SMT, 11.13ng/g for SDZ and 245.91ng/g for SMX, which was higher than the European Union (EU) standard (100ng/g). Moreover, all samples contaminated with SMX also contained SMT, indicating that more than one antibiotic was used for production in the country of origin. Because Thai standards for antibiotics in food have not been completely set, all contaminated discovered would not be considered to be an illegal food, in which antibiotic residues may affect human health in the long term. Therefore, antibiotic residues in Pangasius catfish products should be continually regulated and monitored.

Keywords: ASEAN countries; Food safety; Freshwater fish; LC-MS/MS; Pangasius catfish products; Sulfonamide antibiotics; Thailand.

MeSH terms

Animals
Anti-Bacterial Agents / analysis*
Catfishes
Chromatography, Liquid / methods
Fish Products / analysis*
Food Contamination / analysis*
Hazard Analysis and Critical Control Points / methods*
Sulfadiazine / analysis
Sulfameter / analysis
Sulfamethazine / analysis
Sulfamethoxazole / analysis
Sulfonamides / analysis*
Tandem Mass Spectrometry / methods*
Thailand

Substances

Anti-Bacterial Agents
Sulfonamides
Sulfadiazine
Sulfameter
Sulfamethazine
Sulfamethoxazole