Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodeling Enzymes

C Y Zhou; G J Narlikar

doi:10.1016/bs.mie.2016.01.015

Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodeling Enzymes

Methods Enzymol. 2016:573:119-35. doi: 10.1016/bs.mie.2016.01.015. Epub 2016 Mar 24.

Authors

C Y Zhou¹, G J Narlikar²

Affiliations

¹ University of California, San Francisco, CA, United States.
² University of California, San Francisco, CA, United States. Electronic address: geeta.narlikar@ucsf.edu.

PMID: 27372751
DOI: 10.1016/bs.mie.2016.01.015

Abstract

ATP-dependent chromatin remodeling complexes carry out diverse transformations of chromatin. Understanding their mechanisms requires assays that can monitor the kinetics or chromatin remodeling. In this chapter, we describe complimentary native gel-based and FRET-based methods for assaying the kinetics of ATP-driven nucleosome sliding. These methods can be readily adapted to investigate other types of nucleosomal transformations carried out by chromatin remodeling ATPases.

Keywords: Chromatin remodeling; Enzyme kinetics; Fluorescence resonance energy transfer; Nucleosome labeling.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Adenosine Triphosphatases / metabolism*
Adenosine Triphosphate / metabolism
Animals
Chromatin Assembly and Disassembly*
Fluorescence Resonance Energy Transfer / methods*
Kinetics
Nucleosomes / metabolism*
Xenopus

Substances

Nucleosomes
Adenosine Triphosphate
Adenosine Triphosphatases

Grants and funding

R01 GM073767/GM/NIGMS NIH HHS/United States