Detection of Cell Proliferation Markers by Immunofluorescence Staining and Microscopy Imaging in Paraffin-Embedded Tissue Sections

Curr Protoc Mol Biol. 2016 Jul 1:115:14.25.1-14.25.14. doi: 10.1002/cpmb.13.

Abstract

This unit describes a step-by-step protocol to detect and quantify proliferating cells in paraffin-embedded tissue sections. Two well-established markers of proliferation (incorporation of BrdU into newly synthesized DNA and expression of the nuclear protein Ki67) are detected after antigen-retrieval and subsequent immunofluorescence staining and confocal microscopy. © 2016 by John Wiley & Sons, Inc.

Keywords: BrdU; Ki67; cell proliferation; immunofluorescence; immunohistochemistry.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bromodeoxyuridine / analysis*
  • Cell Proliferation*
  • Fluorescent Antibody Technique / methods*
  • Ki-67 Antigen / analysis*
  • Mice
  • Microscopy, Fluorescence / methods*
  • Paraffin Embedding / methods

Substances

  • Ki-67 Antigen
  • Bromodeoxyuridine