Integrated fabrication-conjugation methods for polymeric and hybrid microparticles for programmable drug delivery and biosensing applications

Abstract

Functionalized polymeric microparticles possess significant potential for controlled drug delivery and biosensing applications, yet current fabrication techniques face challenges in simple and scalable fabrication and biofunctionalization. For programmable manufacture of biofunctional microparticles in a simple manner, we have developed robust micromolding methods combined with biopolymeric conjugation handles and bioorthogonal click reactions. In this focused minireview, we present detailed methods for our integrated approaches for fabrication of microparticles with controlled 2D and 3D shapes and dimensions toward controlled release, and for biomacromolecular conjugation via strain promoted alkyne-azide cycloaddition (SPAAC) and tetrazine-trans-cyclooctene (Tz-TCO) ligation reactions utilizing a potent aminopolysaccharide chitosan as an efficient conjugation handle. We believe that the fabrication-conjugation methods reported here from a range of our recent reports illustrate the simple, robust and readily reproducible nature of our approaches to creating multifaceted microparticles in a programmable, cost-efficient and scalable manner toward a wide range of medical and biotechnological application areas.

Keywords: Bioorthogonal conjugation; Biosensing; Drug delivery; Micromolding; Polymeric microparticles.