Hypersaline phenol-rich wastewater is hard to be treated by traditional biological systems. In this work, a sequencing batch reactor was used to remove phenol from hypersaline wastewater. The removal performance was evaluated in response to the variations of operating parameters and the microbial diversity was investigated by 454 pyrosequencing. The results showed that the bioreactor had high removal efficiency of phenol and was able to keep stable with the increase of initial phenol concentration. DO, pH, and salinity also affected the phenol removal rate. The most abundant bacterial group was phylum Proteobacteria in the two working conditions, and class Gammaproteobacteria as well as Alphaproteobacteria was predominant subgroup. The abundance of bacterial clusters was notably different along with the variation of operation conditions, resulting in changes of phenol degradation rates. The high removal efficiency of phenol suggested that the reactor might be promising in treating phenol-laden industrial wastewater in high-salt condition.
Keywords: 454 pyrosequencing; Biodegradation; High-salt condition; Phenol-containing wastewater; Sequencing batch reactor.
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