Whole-Genome Sequencing Elucidates Epidemiology of Nosocomial Clusters of Acinetobacter baumannii

Stefanie Willems; Stefanie Kampmeier; Stefan Bletz; Annelene Kossow; Robin Köck; Frank Kipp; Alexander Mellmann

doi:10.1128/JCM.00721-16

Whole-Genome Sequencing Elucidates Epidemiology of Nosocomial Clusters of Acinetobacter baumannii

J Clin Microbiol. 2016 Sep;54(9):2391-4. doi: 10.1128/JCM.00721-16. Epub 2016 Jun 29.

Authors

Stefanie Willems¹, Stefanie Kampmeier¹, Stefan Bletz¹, Annelene Kossow¹, Robin Köck², Frank Kipp¹, Alexander Mellmann³

Affiliations

¹ Institute of Hygiene, University Hospital Muenster, Muenster, Germany.
² Institute of Medical Microbiology, University Hospital Muenster, Muenster, Germany.
³ Institute of Hygiene, University Hospital Muenster, Muenster, Germany mellmann@uni-muenster.de.

Abstract

We characterized two epidemiologically similar Acinetobacter baumannii clusters from two separate intensive care units (ICU) using core genome multilocus sequence typing. Clonal spread was confirmed in ICU-1 (12 of 14 isolates shared genotypes); in ICU-2, all genotypes (13 isolates) were diverse, thus excluding transmissions and enabling adequate infection control measures.

MeSH terms

Acinetobacter Infections / epidemiology*
Acinetobacter Infections / microbiology
Acinetobacter baumannii / classification*
Acinetobacter baumannii / genetics*
Acinetobacter baumannii / isolation & purification
Cluster Analysis
Cross Infection / epidemiology*
Cross Infection / microbiology
Genome, Bacterial*
Humans
Intensive Care Units
Molecular Epidemiology / methods*
Multilocus Sequence Typing / methods*

Grants and funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.