Delivery of siRNA Using Cationic Liposomes Incorporating Stearic Acid-modified Octa-Arginine

Anticancer Res. 2016 Jul;36(7):3271-6.

Abstract

Cationic liposomes incorporating stearic acid-modified octa-arginine (StA-R8) were evaluated for survivin small interfering RNA (siRNA) delivery. StA-R8 was synthesized and incorporated into liposomes. The composition of liposomes was optimized. Physicochemical properties, cytotoxicity, cellular uptake and gene silencing activity of the liposomes complexed to survivin siRNA were investigated. The results showed that StA-R8-containing liposomes had reduced cytotoxicity and improved delivery efficiency of siRNA into cancer cells compared with StA-R8 by itself.

Keywords: Cell-penetrating peptide; cancer; cationic liposomes; octa-arginine; survivin.

MeSH terms

  • Cell Line, Tumor
  • Flow Cytometry / methods
  • HEK293 Cells
  • HeLa Cells
  • Hep G2 Cells
  • Humans
  • Inhibitor of Apoptosis Proteins / genetics*
  • Liposomes / administration & dosage
  • Liposomes / chemistry
  • Oligopeptides / administration & dosage*
  • Oligopeptides / chemistry*
  • RNA, Small Interfering / administration & dosage*
  • RNA, Small Interfering / chemistry*
  • RNA, Small Interfering / genetics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
  • Stearic Acids / administration & dosage*
  • Stearic Acids / chemistry*
  • Survivin
  • Transfection / methods

Substances

  • BIRC5 protein, human
  • Inhibitor of Apoptosis Proteins
  • Liposomes
  • Oligopeptides
  • RNA, Small Interfering
  • Stearic Acids
  • Survivin
  • octa-arginine peptide
  • stearic acid