Combination of different methods for detection of Campylobacter spp. in young children with moderate to severe diarrhea

J Microbiol Methods. 2016 Sep:128:7-9. doi: 10.1016/j.mimet.2016.06.026. Epub 2016 Jun 24.

Abstract

Campylobacter spp. were detected - using culture, ELISA, PCR, and qPCR - among children (0-36months) with moderate to severe diarrhea in Northeastern Brazil. Our data showed that either the qPCR alone or PCR along with ELISA might be an alternative to culture to diagnose Campylobacter due to their enhanced sensitivity.

Keywords: Campylobacter spp.; Childhood diarrhea; Disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brazil
  • Campylobacter / isolation & purification*
  • Child, Preschool
  • Diarrhea / diagnosis*
  • Diarrhea / microbiology
  • Enzyme-Linked Immunosorbent Assay*
  • Female
  • Humans
  • Infant
  • Male
  • Real-Time Polymerase Chain Reaction*
  • Sensitivity and Specificity