Chromosomal localisation of the CD4cre transgene in B6·Cg-Tg(Cd4-cre)1Cwi mice

J Immunol Methods. 2016 Sep:436:54-7. doi: 10.1016/j.jim.2016.06.005. Epub 2016 Jun 23.

Abstract

The B6·Cg-Tg(Cd4-cre)1Cwi line expresses CRE recombinase under the control of the promoter and regulatory elements of the Cd4 gene. Here we show that CRE recombinase expression reduces the number and frequencies of CD4 positive subsets in a dose-dependent manner and localize the integration site of the transgenic construct to position 60335693-60341285 (qD) of chromosome 3. The insert contains at least 15 complete sequential copies of the transgenic construct. Based on this information we describe a novel PCR assay for genetic typing of transgenic mice.

Keywords: CD4 cre; CRE recombinase; CRE toxicity; Genotyping; Transgene localisation; Whole genome sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / immunology*
  • Genotype
  • Integrases / genetics*
  • Mice
  • Mice, Transgenic
  • Polymerase Chain Reaction / methods*
  • Promoter Regions, Genetic*
  • Transgenes*

Substances

  • Cre recombinase
  • Integrases