Transcriptional regulation with CRISPR-Cas9: principles, advances, and applications

Andriy Didovyk; Bartłomiej Borek; Lev Tsimring; Jeff Hasty

doi:10.1016/j.copbio.2016.06.003

Transcriptional regulation with CRISPR-Cas9: principles, advances, and applications

Curr Opin Biotechnol. 2016 Aug:40:177-184. doi: 10.1016/j.copbio.2016.06.003. Epub 2016 Jun 23.

Authors

Andriy Didovyk¹, Bartłomiej Borek¹, Lev Tsimring², Jeff Hasty³

Affiliations

¹ BioCircuits Institute, University of California San Diego, La Jolla, CA 92093, USA; San Diego Center for Systems Biology, University of California San Diego, La Jolla, CA 92093, USA.
² BioCircuits Institute, University of California San Diego, La Jolla, CA 92093, USA; San Diego Center for Systems Biology, University of California San Diego, La Jolla, CA 92093, USA. Electronic address: ltsimring@ucsd.edu.
³ BioCircuits Institute, University of California San Diego, La Jolla, CA 92093, USA; San Diego Center for Systems Biology, University of California San Diego, La Jolla, CA 92093, USA; Department of Bioengineering, University of California San Diego, La Jolla, CA 92093, USA; Molecular Biology Section, Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA. Electronic address: jhasty@eng.ucsd.edu.

Abstract

CRISPR-Cas9 has recently emerged as a promising system for multiplexed genome editing as well as epigenome and transcriptome perturbation. Due to its specificity, ease of use and highly modular programmable nature, it has been widely adopted for a variety of applications such as genome editing, transcriptional inhibition and activation, genetic screening, DNA localization imaging, and many more. In this review, we will discuss non-editing applications of CRISPR-Cas9 for transcriptome perturbation, metabolic engineering, and synthetic biology.

Publication types

Review

MeSH terms

Animals
CRISPR-Cas Systems / genetics*
Gene Editing*
Gene Expression Regulation*
Humans
Transcription, Genetic*

Abstract

Publication types

MeSH terms

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