Flow-cytometric analysis of reactive oxygen species in cancer cells under treatment with brassinosteroids

Pyotr A Kisselev; Olesya V Panibrat; Aliaksei R Sysa; Marina V Anisovich; Vladimir N Zhabinskii; Vladimir A Khripach

doi:10.1016/j.steroids.2016.06.010

Flow-cytometric analysis of reactive oxygen species in cancer cells under treatment with brassinosteroids

Steroids. 2017 Jan:117:11-15. doi: 10.1016/j.steroids.2016.06.010. Epub 2016 Jun 23.

Authors

Pyotr A Kisselev¹, Olesya V Panibrat¹, Aliaksei R Sysa¹, Marina V Anisovich¹, Vladimir N Zhabinskii², Vladimir A Khripach¹

Affiliations

¹ Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich str., 5/2, 220141 Minsk, Belarus.
² Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich str., 5/2, 220141 Minsk, Belarus. Electronic address: vz@iboch.bas-net.by.

PMID: 27343978
DOI: 10.1016/j.steroids.2016.06.010

Abstract

To explore the underlying mechanism of cancer cell growth inhibition by brassinosteroids (BS), reactive oxygen species (ROS) generation under treatment with 28-homocastasterone and its synthetic derivatives (22S,23S)-28-homocastasterone was measured in A549 human lung adenocarcinoma cells. BS induced ROS generation in A549 cells and their growth in a time and dose-dependent manner. The maximal effect was observed for (22S,23S)-28-homocastasterone which at 30μM concentration showed a 6-fold increase of ROS generation in comparison with the control.

Keywords: 28-Homocastasterone; Anticancer; Brassinosteroids; Cancer cell line A549; Reactive oxygen species.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

A549 Cells
Antineoplastic Agents / pharmacology*
Brassinosteroids / pharmacology*
Cell Cycle / drug effects
Cell Line, Tumor
Cell Survival / drug effects*
Flow Cytometry
Humans
Reactive Oxygen Species / metabolism*

Substances

Antineoplastic Agents
Brassinosteroids
Reactive Oxygen Species