Improving enzymatic hydrolysis of lignocellulosic substrates with pre-hydrolysates by adding cetyltrimethylammonium bromide to neutralize lignosulfonate

Cheng Cai; Xueqing Qiu; Xuliang Lin; Hongming Lou; Yuxia Pang; Dongjie Yang; Siwei Chen; Kaifan Cai

doi:10.1016/j.biortech.2016.06.043

Improving enzymatic hydrolysis of lignocellulosic substrates with pre-hydrolysates by adding cetyltrimethylammonium bromide to neutralize lignosulfonate

Bioresour Technol. 2016 Sep:216:968-75. doi: 10.1016/j.biortech.2016.06.043. Epub 2016 Jun 16.

Authors

Cheng Cai¹, Xueqing Qiu², Xuliang Lin¹, Hongming Lou³, Yuxia Pang¹, Dongjie Yang¹, Siwei Chen¹, Kaifan Cai¹

Affiliations

¹ School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
² School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China.
³ School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China. Electronic address: cehmlou@scut.edu.cn.

PMID: 27343448
DOI: 10.1016/j.biortech.2016.06.043

Abstract

Two pretreatment methods to overcome recalcitrance of lignocelluloses, sulfite pretreatment (SPORL) and dilute acid (DA), were conducted to pretreat softwood masson pine and hardwood eucalyptus for enzymatic hydrolysis. In the presence of corresponding pre-hydrolysates, adding moderate cetyltrimethylammonium bromide (CTAB) could enhance the enzymatic hydrolysis of the SPORL-pretreated substrates, but had no enhancement for the DA-pretreated substrates. The results showed that sodium lignosulfonate (SL) in pre-hydrolysates and CTAB together had a strong enhancement on the enzymatic hydrolysis of lignocelluloses. The compound of commercial lignosulfonate SXSL and CTAB (SXSL-CTAB) could enhance the substrate enzymatic digestibility (SED) of SPORL-pretreated masson pine from 27.1% to 71.0%, and that of DA-pretreated eucalyptus from 37.6% to 67.9%. The mechanism that CTAB increased the adsorption of SL on lignin to form more effective steric hindrance and reduced the non-productive adsorption of cellulase on lignin by neutralizing the negative charge of SL was proposed.

Keywords: Cellulase; Cetyltrimethylammonium bromide; Enzymatic hydrolysis; Lignocellulose; Lignosulfonate.

MeSH terms

Adsorption
Cellulase / chemistry
Cetrimonium
Cetrimonium Compounds / chemistry*
Eucalyptus / chemistry
Hydrolysis
Lignin / analogs & derivatives*
Lignin / chemistry
Lignin / metabolism
Pinus / chemistry
Sulfites / chemistry
Wood / chemistry*

Substances

Cetrimonium Compounds
Sulfites
lignocellulose
lignosulfuric acid
Lignin
Cellulase
Cetrimonium